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Literature summary for 2.1.1.250 extracted from

  • Creighbaum, A.J.; Ticak, T.; Shinde, S.; Wang, X.; Ferguson, D.J.
    Examination of the glycine betaine-dependent methylotrophic methanogenesis pathway insights into anaerobic quaternary amine methylotrophy (2019), Front. Microbiol., 10, 2572 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.250

Cloned(Commentary)

Cloned (Comment) Organism
gene mtgB or MV8460, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Methanolobus vulcani

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Methanolobus vulcani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] Methanolobus vulcani
-
 a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] Methanolobus vulcani B1
-
 a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] Methanolobus vulcani B1d
-
 a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?

Organism

Organism UniProt Comment Textmining
Methanolobus vulcani
-
-
-
Methanolobus vulcani B1d
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information proteomics analysis Methanolobus vulcani
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme MV8460 shows GB:cob(I)alamin methyl-transfer activity. Molecular docking Methanolobus vulcani ?
-
-
additional information enzyme MV8460 shows GB:cob(I)alamin methyl-transfer activity. Molecular docking Methanolobus vulcani B1 ?
-
-
additional information enzyme MV8460 shows GB:cob(I)alamin methyl-transfer activity. Molecular docking Methanolobus vulcani B1d ?
-
-
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
-
 Methanolobus vulcani a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
-
 Methanolobus vulcani B1 a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
-
 Methanolobus vulcani B1d a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?

Synonyms

Synonyms Comment Organism
MtgB
-
 Methanolobus vulcani
MV8460
-
 Methanolobus vulcani
MV8460 GB:cob(I)alamin
-
 Methanolobus vulcani
non-pyrrolysine MttB homolog
-
 Methanolobus vulcani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanolobus vulcani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Methanolobus vulcani

General Information

General Information Comment Organism
evolution the enzyme belongs to the COG5598 MttB superfamily. Based on the draft B1d genome, MV8460 is the sole Pyl-lacking MttB found in B1d, which is 65% identical and has 83% sequence similarity to Desulfitobacterium hafniense Y51 DhMtgB. Four predicted active site residues may interact with GB as they are conserved between DhMtgB and MV8460: Y94, N199, R309, and H345 Methanolobus vulcani
metabolism the enzyme is involved in the the glycine betaine-dependent methylotrophic methanogenesis pathway. Environmentally abundant quaternary amines (QAs) are a primary source for methanogenesis. The methanogenic archaeon Methanolobus vulcani B1d metabolizes glycine betaine (GB) through a corrinoid-dependent GB:coenzyme M (CoM) methyl transfer pathway Methanolobus vulcani
physiological function MV8460 catalyzes GB-dependent methylation of free cob(I) alamin indicating it is an authentic MtgB enzyme Methanolobus vulcani